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1 article(s) from Boutorine, Alexandre S

Application of Cu(I)-catalyzed azide–alkyne cycloaddition for the design and synthesis of sequence specific probes targeting double-stranded DNA

Svetlana V. Vasilyeva,
Vyacheslav V. Filichev and
Alexandre S. Boutorine

Beilstein J. Org. Chem. 2016, 12, 1348–1360, doi:10.3762/bjoc.12.128

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Graphical Abstract

Figure 1: A) Formation of nucleotide triplets in parallel and antiparallel (relatively to polypurine strand) ...

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Figure 2: Synthesis of MGB-fluorophore (A) and MGB-TFO (B) conjugates using CuACC. Linker length and composit...

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Figure 3: Bifunctional linkers for conjugation of oligonucleotides and polyamides using CuACC.

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Figure 4: The target duplex contains a 29 base pair fragment from HIV proviral DNA [35] and a T4 hairpin is conne...

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Figure 5: A) Sequence derived from the murine pericentromere repeat fragment with only one target site for th...

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Figure 6: Synthesis of azide- and alkyne-modified MGBs.

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Figure 7: Structures of fluorescent probes synthesized by "click chemistry".

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Figure 8: Titration of the probes F1-NH₂-MM14 (12 µM, A, C) and F1-NH₂-TO (10 µM, B, D) by the target DNA dup...

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Figure 9: Synthesis of modified oligonucleotides containing an alkyne group.

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Figure 10: Gel electrophoresis of oligonucleotides modified by alkyne linkers: A – oligonucleotide HIVP (detec...

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Figure 11: TINA-TFOs bearing a 3'-alkyne group for antiparallel triplex formation with the target HIV proviral...

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Figure 12: Structures of polyamide-TFO conjugates.

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Figure 13: Electrophoresis analysis of samples from reaction mixtures after click reactions between alkyne-TFO...

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Figure 14: Electrophoresis analysis of reaction mixtures in 20% denaturing polyacrylamide gel after TINA-TFO-M...

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Figure 15: Electrophoretic analysis of reaction mixtures in standard 20% denaturing PAGE after DNA-templated s...

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Figure 16: Non-denaturing gel electrophoresis analysis of conjugate 28 with fluorescein-labeled target HIV dup...

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Published 30 Jun 2016

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