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Beilstein J. Org. Chem. 2016, 12, 1348–1360, doi:10.3762/bjoc.12.128
Graphical Abstract
Figure 1: A) Formation of nucleotide triplets in parallel and antiparallel (relatively to polypurine strand) ...
Figure 2: Synthesis of MGB-fluorophore (A) and MGB-TFO (B) conjugates using CuACC. Linker length and composit...
Figure 3: Bifunctional linkers for conjugation of oligonucleotides and polyamides using CuACC.
Figure 4: The target duplex contains a 29 base pair fragment from HIV proviral DNA [35] and a T4 hairpin is conne...
Figure 5: A) Sequence derived from the murine pericentromere repeat fragment with only one target site for th...
Figure 6: Synthesis of azide- and alkyne-modified MGBs.
Figure 7: Structures of fluorescent probes synthesized by "click chemistry".
Figure 8: Titration of the probes F1-NH2-MM14 (12 µM, A, C) and F1-NH2-TO (10 µM, B, D) by the target DNA dup...
Figure 9: Synthesis of modified oligonucleotides containing an alkyne group.
Figure 10: Gel electrophoresis of oligonucleotides modified by alkyne linkers: A – oligonucleotide HIVP (detec...
Figure 11: TINA-TFOs bearing a 3'-alkyne group for antiparallel triplex formation with the target HIV proviral...
Figure 12: Structures of polyamide-TFO conjugates.
Figure 13: Electrophoresis analysis of samples from reaction mixtures after click reactions between alkyne-TFO...
Figure 14: Electrophoresis analysis of reaction mixtures in 20% denaturing polyacrylamide gel after TINA-TFO-M...
Figure 15: Electrophoretic analysis of reaction mixtures in standard 20% denaturing PAGE after DNA-templated s...
Figure 16: Non-denaturing gel electrophoresis analysis of conjugate 28 with fluorescein-labeled target HIV dup...